Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Free cholesterol fiber-optic biosensor for serum samples with simplex optimization

M D Marazuela1, B Cuesta, M C Moreno-Bondi

  • 1Department of Analytical Chemistry, Complutense University, Madrid, Spain.

Biosensors & Bioelectronics
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of alternariol and alternariol monomethyl ether in foodstuffs by molecularly imprinted solid-phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry.

Food chemistry·2017
Same author

Comparison of sample preparation strategies for target analysis of total thyroid hormones levels in serum by liquid chromatography-quadrupole time-of-flight-mass spectrometry.

Talanta·2017
Same author

Multiresidue analysis of cephalosporin antibiotics in bovine milk based on molecularly imprinted polymer extraction followed by liquid chromatography-tandem mass spectrometry.

Journal of chromatography. A·2016
Same author

Multiresidue determination of fluoroquinolone antimicrobials in baby foods by liquid chromatography.

Food chemistry·2014
Same author

Molecular recognition with nanostructures fabricated by photopolymerization within metallic subwavelength apertures.

Nanoscale·2014
Same author

Multiresidue analysis of fluoroquinolone antimicrobials in chicken meat by molecularly imprinted solid-phase extraction and high performance liquid chromatography.

Journal of chromatography. A·2014
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026
Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026
Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026
Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026
Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026
See all related articles

This study introduces an optical fiber biosensor for measuring free cholesterol in serum. The novel sensor utilizes a ruthenium complex and cholesterol oxidase, demonstrating a dynamic range of 0.15-3.0 mM for accurate cholesterol monitoring.

Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Materials Science

Background:

  • Accurate free cholesterol monitoring in serum is crucial for diagnosing and managing cardiovascular diseases.
  • Existing biosensors often face challenges with sensitivity, stability, and complex sample matrices.
  • Optical biosensors offer potential advantages due to their non-invasive nature and real-time detection capabilities.

Purpose of the Study:

  • To develop and optimize an optical fiber biosensor for the quantitative detection of free cholesterol in serum samples.
  • To investigate the sensor's performance characteristics, including dynamic range, reproducibility, and stability.
  • To validate the sensor's applicability for real-world clinical diagnostics.

Main Methods:

  • Fabrication of an optical fiber biosensor using silicone-entrapped tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) complex as an optical transducer.

Related Experiment Videos

  • Immobilization of cholesterol oxidase within a graphite powder layer on a dyed silicone film.
  • Optimization of interdependent chemical variables using a super-modified simplex method.
  • Characterization of sensor performance, including dynamic range, reproducibility, stability, and interference studies.

    • Main Results:

    • The developed optical fiber biosensor exhibits a dynamic range of 0.15-3.0 mM for free cholesterol.
    • The sensor demonstrates good reproducibility and stability over time.
    • Successful application of the sensor for free cholesterol measurements in actual serum samples was achieved.
    • Simplex optimization proved effective in identifying optimal analytical conditions.

    Conclusions:

    • The developed optical fiber biosensor provides a sensitive and reliable method for free cholesterol monitoring in serum.
    • The sensor design, integrating a ruthenium complex transducer and enzymatic detection, shows promise for clinical applications.
    • Simplex optimization is a valuable tool for enhancing the performance of optical biosensors.