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Structure and expression of the mouse S10 gene
1Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Biochimica Et Biophysica Acta
|March 20, 1997
Summary
Researchers cloned mouse S10 cDNA, revealing high similarity to human S10. The mouse S10 gene has a simple structure and is primarily expressed in the brain.
Area of Science:
- Molecular Biology
- Genetics
- Neuroscience
Background:
- The S10 gene encodes a small GTP-binding protein within the Rab subfamily.
- Previous work reported the cloning of the human S10 cDNA.
Purpose of the Study:
- To describe the mouse S10 cDNA sequence.
- To elucidate the genomic structure of the mouse S10 gene.
- To investigate the expression pattern of the mouse S10 gene.
Main Methods:
- cDNA cloning and sequencing.
- Genomic DNA analysis.
- Northern blotting of tissue RNA.
Main Results:
- The mouse S10 cDNA sequence was determined.
- Mouse S10 exhibits 92.3% nucleotide and 98.3% amino acid homology to human S10.
- The mouse S10 gene consists of two exons and one intron.
- Northern blotting revealed predominant S10 gene expression in the brain.
Conclusions:
- Mouse S10 is highly homologous to human S10, suggesting conserved function.
- The mouse S10 gene possesses a simple exon-intron structure.
- The brain is the primary site of S10 gene expression in mice.