Related Experiment Videos
Application of suppression PCR to the megaprimer method for site-directed mutagenesis
S Kaluz1, M Kaluzova, A P Flint
1Department of Physiology and Environmental Science, University of Nottingham, Loughborough, UK.sbzsk@sbn3.nott.AC.UK
Genetic Analysis : Biomolecular Engineering
|December 1, 1996
Abstract:
Combination of suppression polymerase chain reaction (PCR) and megaprimer method of site-directed mutagenesis allows specific reamplification of extended megaprimer. This modification is proposed for templates that are difficult to amplify when standard megaprimer procedures do not generate sufficient yield of mutated product.