[Influence of chlorpromazine and temperature on glucose transport in human erythrocyte ghosts]

Glucose transport was determined by oxygen adsorption in solution when glucose was oxidized with glucoseoxidase, injected into erythrocyte ghosts. Temperature dependence of glucose transport velocity shows a break at 20 degrees C, which was described in literature. Small concentrations of chlorpromazine (about 2-10(-5) M) somewhat activates the transport but does not changes its temperature dependence. Higher concentrations of anaesthetics (beginning with 1-10(-4) M) inhibit the transport, decrease the temperature of break and increase the activation energyies of the process both lower and higher of the transition temperature. The change of working velocity of glucose carrier under the action of anaesthetic and temperature is accounted for by structural reconstructions in lipids, which are heterogeneously distributed along the erythrocyte membrane plane.