[An experimental model of acute purulent peritonitis]

There are important shortcomings in all the existing models of acute peritonitis. The original model, most close to clinical conditions, has been proposed. The grey rabbits of 1.5-2.0 kg were used in experimental trials. The 5 cm laparotomy has been performed in all the rabbits under thiopental anesthesia. In rabbits of the control group the wound was closed without any manipulations in abdominal cavity. In the other animals the terminal part of the small intestine (15-18 cm) was isolated with two intestinal forceps and saline solution was injected inside. The intestine content was aspirated and mixed with the rabbits blood. The punctate without blood was injected in rabbits of the 2d group; in the 3d group-the mixture of punctate and blood (5 ml of blood per kg of body weight); 10 ml per kg were injected in rabbits of the 4th group; 15 ml per body weight-in the 5th group. In rabbits of the 1st, 2d and 3d groups there were no signs of peritonitis 7 days after the injection. In animals of the 4th and 5th groups clinical, morphological and bacteriological sings of acute peritonitis were detected. The severity of its course depended on the amount of blood in the mixture that was used for irritation of peritoneum. All animals of the 5th group died by the end of the 4th day. Thus, the use of 10 ml of autopunctuate of terminal part of small intestine in combination with the animals blood (10 ml per kg), is the most proper model of peritonitis, that is most close to clinical situations and enables to study its phases and test various methods of treatment.