Overexpression of prothymosin alpha, concomitant with c-myc, during rat hepatic carcinogenesis

By using a subtraction-enhanced display technique, we identified a cDNA clone representing alpha-prothymosin, from rat hepatocellular carcinoma (HCC). alpha-Prothymosin has been reported to be involved in cell proliferation and regulated by c-myc gene in vitro. In the present study, we investigated the gene expression pattern of alpha-prothymosin and analyzed its correlation with c-myc during rat hepatic carcinogenesis and liver regeneration. Hepatic alpha-prothymosin mRNA levels, concomitant with c-myc, were increased at the early stage of hepatic carcinogenesis (6 weeks), and remained nearly 10 fold higher as the tumor progressed. In comparison, alpha-prothymosin mRNA levels were only slightly and moderately increased at early (3-6 hr) and at later stage (24-30 hr) of liver regeneration after 70% partial hepatectomy. In situ hybridization revealed that overexpressed alpha-prothymosin mRNA was restricted to the tumor nodules and to tumor cells invading blood vessels. These data provide evidence that overexpression of alpha-prothymosin, concomitant with c-myc, is related to rat hepatic carcinogenesis.