Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A blind testing design for authenticating ancient DNA sequences

H Yang1, E M Golenberg, J Shoshani

  • 1Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA. hongyang@umich.edu

Molecular Phylogenetics and Evolution
|April 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Foraging strategy in a subterranean rodent, Spalax ehrenbergi: a test case for optimal foraging theory.

Oecologia·2017
Same author

Genetic diversity and response to IFN of the NS3 protease gene from clinical strains of the hepatitis C virus.

Archives of virology·2002
Same author

The Mycobacterium tuberculosis small heat shock protein Hsp16.3 exposes hydrophobic surfaces at mild conditions: conformational flexibility and molecular chaperone activity.

Protein science : a publication of the Protein Society·1999
Same author

Antagonistic actions of Arabidopsis cryptochromes and phytochrome B in the regulation of floral induction.

Development (Cambridge, England)·1999
Same author

Intracisternal TRH analog increases gastrin release and corpus histidine decarboxylase activity in rats.

The American journal of physiology·1999
Same author

Co-encapsulation of Sertoli enriched testicular cell fractions further prolongs fish-to-mouse islet xenograft survival.

Transplantation·1999
Same journal

Adaptive introgression facilitates tropical plant evolution: Pandanus as a case study.

Molecular phylogenetics and evolution·2026
Same journal

Molecular phylogenetics of Andean killifish genus Orestias (Cyprinodontiformes: Orestiidae) with emphasis on the O. agassii species complex, a widespread Andean clade.

Molecular phylogenetics and evolution·2026
Same journal

Resolving fuzzy species limits: nuclear evidence for speciation by hybridisation in Amazonian Trichomanes ferns.

Molecular phylogenetics and evolution·2026
Same journal

Phylogenomic assessment of microhylid frogs reveals widespread taxonomic confusion in the Asterophryinae and establishes the timing of diversification in Australia.

Molecular phylogenetics and evolution·2026
Same journal

Phylogenomics, introgression, and demographic history of extant flamingos (Aves: Phoenicopteridae).

Molecular phylogenetics and evolution·2026
Same journal

The biogeographic and evolutionary history of Leiosauridae lizards reveal the influence of complex environmental and geological past dynamics in the Neotropical biomes.

Molecular phylogenetics and evolution·2026
See all related articles

Blind testing enhances ancient DNA research reliability. This method validates laboratory accuracy and sequence authenticity, ensuring trustworthy phylogenetic analysis for ancient specimens.

Area of Science:

  • Paleogenomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Reproducibility is a significant challenge in ancient DNA (aDNA) research.
  • Ensuring the accuracy and authenticity of aDNA sequences is crucial for reliable scientific conclusions.

Purpose of the Study:

  • To design and implement a blind testing procedure to evaluate laboratory accuracy and authenticity of ancient DNA.
  • To assess the reliability of aDNA results using a novel blind testing approach.

Main Methods:

  • A blind testing protocol was developed using soft tissue and bone samples from proboscideans (extinct and extant).
  • Samples were morphologically identified by one researcher and DNA tested by others under anonymous coding.
  • DNA was extracted using three methods, amplified for a cytochrome b gene segment, and sequenced.

Related Experiment Videos

Main Results:

  • Phylogenetic analysis of the blind-tested ancient DNA sequences demonstrated consistency and reliability.
  • The blind testing procedure, when combined with phylogenetic analysis, provided a nonarbitrary method for evaluating aDNA results.
  • Reproducible results increased confidence in the authenticity of ancient sequences.

Conclusions:

  • Blind testing is an effective method for validating laboratory accuracy and authenticity in ancient DNA studies.
  • This approach can increase confidence in ancient sequence data and mitigate bias in phylogenetic analyses.
  • Blind testing offers a viable alternative for confirming aDNA results when independent laboratory verification is not feasible.