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Related Experiment Videos

Hemoglobin interference with Du Pont Automatic Clinical Analyzer procedure for calcium

W H Porter, J R Carroll, R E Roberts

    Clinical Chemistry
    |November 1, 1977
    PubMed
    Summary

    High hemoglobin levels can falsely elevate serum calcium measurements. This interference, caused by oxyhemoglobin absorption, affects accuracy in clinical diagnostics.

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    Area of Science:

    • Clinical Chemistry
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate serum calcium measurement is vital for diagnosing various medical conditions.
    • Hemoglobin is a common component in blood samples, and its properties can potentially interfere with analytical assays.

    Purpose of the Study:

    • To investigate the interference of hemoglobin on serum calcium determination using the Du Pont aca analyzer.
    • To identify the specific mechanism behind this observed interference.

    Main Methods:

    • Serum samples with varying hemoglobin concentrations were analyzed for calcium using the Du Pont aca.
    • Spectrophotometric analysis was employed to examine the absorption characteristics of oxyhemoglobin.

    Main Results:

    • Hemoglobin concentrations of approximately 2.5 g/liter or greater significantly increased apparent serum calcium values.
    • The interference was attributed to the 575-nm absorption band of oxyhemoglobin.
    • The analyzer's differential absorption reading at 577-600 nm did not compensate for this oxyhemoglobin interference.

    Conclusions:

    • Hemoglobin is a significant source of interference in serum calcium assays performed on the Du Pont aca.
    • Clinical laboratories should be aware of this interference when interpreting calcium results from samples with elevated hemoglobin levels.

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