Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ATPase activity in the breast: a comparison between three methods

J A Quinones, L J van Bogaert

    Acta Histochemica
    |January 1, 1979
    PubMed
    Summary

    Adenosine triphosphatase (ATPase) activity was studied in human breast tissue. ATPase enzymatic activity was detected in myoepithelial cells and blood vessels but not in carcinoma cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Dynamics of allograft fibrosis in pediatric liver transplantation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014
    Same author

    Suggestions for SAMJ editorial policies.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2008
    Same author

    Termination of pregnancy with misoprostol in the scarred uterus.

    International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2007
    Same author

    A comparison of women's, providers' and ultrasound assessments of pregnancy duration among termination of pregnancy clients in South Africa.

    BJOG : an international journal of obstetrics and gynaecology·2007
    Same author

    Opportunistic testing of medically underserved women for cervical cancer in South Africa.

    Acta cytologica·2001
    Same author

    Lumbar lordosis and the spread of subarachnoid hyperbaric 0.5% bupivacaine at cesarean section.

    International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2000

    Area of Science:

    • Biochemistry
    • Histology
    • Oncology

    Background:

    • Adenosine triphosphatase (ATPase) plays a role in cellular energy metabolism.
    • Understanding ATPase activity in mammary tissue is crucial for differentiating normal, dysplastic, and neoplastic states.

    Purpose of the Study:

    • To investigate and compare adenosine triphosphatase (ATPase) enzymatic activity in normal, dysplastic, and neoplastic human mammary tissues.
    • To evaluate the efficacy of three different histochemical methods for detecting ATPase activity.

    Main Methods:

    • Histochemical analysis of adenosine triphosphatase (ATPase) activity using three distinct methods.
    • Comparison of staining intensity and localization across different mammary tissue types (normal, dysplastic, neoplastic).
    • Evaluation of calcium-cobalt and lead-nitrate based staining procedures.

    Main Results:

    • ATPase enzymatic activity was observed in myoepithelial cells and blood vessels of mammary tissue.
    • Epithelial cells showed weak or no ATPase activity; carcinoma cells were consistently unlabeled.
    • The calcium-cobalt method yielded more intense reactions than lead-nitrate methods, with enhanced stain deposit sharpness.

    Conclusions:

    • ATPase activity is differentially expressed in human mammary tissues, with a notable absence in carcinoma cells.
    • Histochemical methods, particularly the calcium-cobalt technique, are effective for visualizing ATPase activity in mammary tissue.
    • Further research into the functional significance of ATPase activity in breast tissue is warranted.

    Related Experiment Videos