Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization and function of the mitochondrial outer membrane peptide-sensitive channel

J P Henry1, P Juin, F Vallette

  • 1Centre National de la Recherche Scientifique, Service de Neurobiologie Physico-Chimique, Institut de Biologie Physico-Chimique, Paris, France.

Journal of Bioenergetics and Biomembranes
|April 1, 1996
PubMed
Summary

The peptide-sensitive channel (PSC) in mitochondria is blocked by peptides, suggesting peptide translocation through the outer membrane. Irreversible peptide interactions may explain PSC property variations and involvement in protein transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Residency training in robotic surgery: The role of simulation.

Journal of visceral surgery·2020
Same author

DNA methylation signal has a major role in the response of human breast cancer cells to the microenvironment.

Oncogenesis·2017
Same author

The role of oxidative damage in poor scalp health: ramifications to causality and associated hair growth.

International journal of cosmetic science·2015
Same author

miR-491-5p-induced apoptosis in ovarian carcinoma depends on the direct inhibition of both BCL-XL and EGFR leading to BIM activation.

Cell death & disease·2014
Same author

An angiosperm-wide analysis of the gynodioecy-dioecy pathway.

Annals of botany·2014
Same author

Bcl-xL controls a switch between cell death modes during mitotic arrest.

Cell death & disease·2014

Area of Science:

  • Mitochondrial biogenesis
  • Membrane transport
  • Molecular biology

Background:

  • The peptide-sensitive channel (PSC) is a large conductance cation channel found in yeast and mammalian mitochondria.
  • Its precise role and localization within the mitochondrial membranes have been areas of ongoing investigation.

Purpose of the Study:

  • To characterize the peptide-sensitive channel (PSC) in mitochondria.
  • To investigate the localization and function of PSC in the outer mitochondrial membrane.
  • To explore the interaction of peptides with PSC and its implications for channel properties and protein translocation.

Main Methods:

  • Electrophysiological characterization using "tip-dip," patch clamp of giant liposomes, and planar bilayers.
  • Analysis of PSC frequency in different mitochondrial fractions.

Related Experiment Videos

  • Mitochondrial uptake measurements using iodinated peptides in a porin-less mutant.

    • Main Results:

    • Yeast and mammalian PSC are blocked by basic peptides, such as pCyt OX IV (1-12)Y, indicating peptide translocation.
    • Electrophysiological data support peptide translocation through the PSC pore.
    • PSC is localized in the outer mitochondrial membrane, facilitating peptide translocation across it.
    • Certain peptides induce irreversible alterations in PSC properties, potentially explaining channel variability.
    • Evidence suggests PSC involvement in protein translocation and interaction with the outer membrane translocation machinery.

    Conclusions:

    • The peptide-sensitive channel (PSC) is a key component of the outer mitochondrial membrane involved in peptide translocation.
    • Irreversible peptide binding to PSC can modulate its function and may contribute to observed variability in channel properties.
    • PSC likely plays a role in mitochondrial protein import and interacts with the general insertion pore.