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Insertionally inactivated and inducible recA alleles for use in Neisseria
1Northwestern University Medical School, Department of Microbiology-Immunology, Chicago, IL 60611, USA. h-seifert@nwu.edu
Gene
|April 1, 1997
Abstract:
Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec- and Rec+ by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.