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Regulation of cyclodextrin glucanotransferase synthesis in Bacillus ohbensis
T Nishida1, A Nakamura, H Masaki
1Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
FEMS Microbiology Letters
|April 15, 1997
Summary
Cyclodextrin glucanotransferase (CGTase) production by Bacillus ohbensis is regulated by starch and glucose. Glucose represses CGTase gene transcription, while starch may influence production post-transcriptionally.
Area of Science:
- Microbiology
- Enzymology
- Molecular Biology
Background:
- Bacillus ohbensis produces cyclodextrin glucanotransferase (CGTase), an enzyme with industrial applications.
- CGTase production is known to be influenced by medium composition, specifically carbon sources.
Purpose of the Study:
- To investigate the regulatory mechanisms controlling CGTase gene expression and protein production in Bacillus ohbensis.
- To elucidate the roles of starch and glucose in the regulation of CGTase synthesis.
Main Methods:
- Northern blot analysis to assess CGTase gene (cgt) transcription levels.
- Western blot analysis to quantify CGTase protein production.
- Cultivation of Bacillus ohbensis in media with varying starch and glucose concentrations.
Main Results:
- CGTase gene transcription was largely unaffected by starch presence but completely repressed by glucose.
- Significant CGTase protein accumulation was observed only in the presence of starch.
- No evidence of post-translational regulation of CGTase activity was found.
Conclusions:
- Glucose exerts negative transcriptional control over the CGTase gene (cgt) in Bacillus ohbensis.
- Starch appears to induce CGTase production through a post-transcriptional regulatory mechanism.
- The findings suggest a complex regulatory network governing CGTase synthesis in this bacterium.