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Intravenous glucose tolerance test in Macaca radiata radiata
K Sathya1, A N Balamurugan, S Savithiri
1Department of Physiology, Christian Medical College, Vellore.
Indian Journal of Physiology and Pharmacology
|April 1, 1997
Summary
A standardized intravenous glucose tolerance test in monkeys reliably assesses beta cell function. This method establishes a normal glucose disposal curve and documents insulin level variability.
Area of Science:
- Endocrinology
- Primate Research
- Metabolic Studies
Background:
- Accurate assessment of beta cell function is crucial for understanding glucose homeostasis.
- Existing methods for glucose tolerance testing in non-human primates can lack sensitivity or reliability.
- Standardization is needed for reproducible studies in primate models of metabolic disease.
Purpose of the Study:
- To develop and standardize a sensitive intravenous glucose tolerance test (IVGTT) protocol for use in monkeys.
- To establish a reference curve for normal glucose disposal in this species.
- To characterize insulin secretion patterns during the IVGTT.
Main Methods:
- Standardization of an intravenous glucose tolerance test (IVGTT) procedure in a monkey cohort.
- Intravenous administration of a glucose load.
- Serial blood sampling for glucose and insulin level measurements.
Main Results:
- A reliable and sensitive IVGTT method for monkeys was successfully standardized.
- A normal curve depicting glucose disposal following the glucose challenge was constructed.
- Significant variability in insulin level responses was observed and documented.
Conclusions:
- The standardized IVGTT provides a reliable tool for assessing beta cell function in monkeys.
- This method facilitates comparative studies of glucose metabolism and insulin secretion in primate models.
- Further investigation into the sources of insulin level variability is warranted.