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Comparison of three different methods for radiolabelling human activated T lymphocytes
C Botti1, D R Negri, E Seregni
1Nuclear Medicine Department, National Cancer Institute, Milano, Italy.
European Journal of Nuclear Medicine
|May 1, 1997
Summary
Indium-111 oxine is the most suitable radiolabel for activated T lymphocytes in ovarian cancer studies. This method allows for effective in vivo biodistribution imaging while maintaining T cell function and viability.
Area of Science:
- Immunology
- Oncology
- Radiochemistry
Background:
- Ovarian cancer treatment utilizes T lymphocytes and bispecific monoclonal antibodies (biMAb OC/TR).
- In vivo biodistribution of these T cells is crucial for treatment efficacy but poorly understood.
- Scintigraphic imaging of radiolabeled T cells can visualize migratory patterns.
Purpose of the Study:
- To compare the efficiency, stability, and toxicity of technetium-99m hexamethylpropylene amine oxime (99mTc-HMPAO), indium-111 oxine (111In-oxine), and fluorine-18 2-fluoro-2-deoxy-d-glucose (18F-FDG) for radiolabeling activated T lymphocytes.
- To determine the optimal radiolabeling reagent for in vivo biodistribution studies of T cells targeted with biMAb OC/TR for ovarian cancer treatment.
Main Methods:
- Activated T lymphocytes targeted with biMAb OC/TR were radiolabeled using 99mTc-HMPAO, 111In-oxine, and 18F-FDG.
- Labeling efficiency, radionuclide retention, cell viability, immunophenotype, cytotoxic activity, and proliferative ability were assessed.
- Comparisons were made between different radiolabelling reagents and cell numbers.
Main Results:
- 111In-oxine and 18F-FDG showed higher labeling efficiencies (68%, 64%) than 99mTc-HMPAO (31%).
- 111In-oxine demonstrated superior radionuclide retention ( < 25% release after 240 min) compared to 99mTc-HMPAO (44% after 240 min) and 18F-FDG (45% after 150 min).
- While cell viability and immunophenotype were unaffected, 111In-oxine and 18F-FDG reduced cytotoxic activity, and all reagents impaired proliferative ability. Using 111In-oxine with a higher cell number improved viability and proliferation.
Conclusions:
- 111In-oxine is the most appropriate reagent for radiolabeling activated T lymphocytes for in vivo biodistribution studies in ovarian cancer.
- Low activity per cell is recommended for 111In-oxine labeling to maintain T cell function.
- This approach aids in visualizing T cell migration patterns for improved targeted cancer therapies.