Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA vaccines

J J Donnelly1, J B Ulmer, J W Shiver

  • 1Department of Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 19486, USA.

Annual Review of Immunology
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA vaccines.

Methods in molecular medicine·2011
Same author

A Modified ELISA for Measurement of High-Avidity IgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers.

Methods in molecular medicine·2011
Same author

The second Geneva Consensus: Recommendations for novel live TB vaccines.

Vaccine·2010
Same author

Public health. The cholera crisis in Africa.

Science (New York, N.Y.)·2009
Same author

Topic in Review: Vaccines in the 21st century.

The Western journal of medicine·2008
Same author

Pre-clinical evaluation of a CEA DNA prime/protein boost vaccination strategy against colorectal cancer.

Scandinavian journal of immunology·2007
Same journal

The Neuroimmune Circuitry of Peripheral Sensory Neuron Subtypes in Chronic Pain.

Annual review of immunology·2026
Same journal

The Spectrum of Fibroblast Immune Biology.

Annual review of immunology·2026
Same journal

The Molecular Logic of Immunoglobulin Heavy Chain Class Switch Recombination.

Annual review of immunology·2026
Same journal

Decoding Human T Cell Immunity with Artificial Intelligence and Single-Cell Genomics.

Annual review of immunology·2026
Same journal

Immune Niches in Cancer.

Annual review of immunology·2026
Same journal

T Cell Receptor Signaling and Immune Tolerance: From Autoimmunity to Cancer Immunity.

Annual review of immunology·2026
See all related articles

DNA immunization uses plasmid DNA injection to induce immune responses against diseases. This novel vaccination method effectively generates antibody and cell-mediated immunity, particularly useful for cytotoxic T cell induction.

Area of Science:

  • Immunology
  • Molecular Biology
  • Vaccinology

Background:

  • Plasmid DNA's ability to transfect animal cells in vivo was observed in the early 1990s.
  • This led to the development of DNA immunization for inducing immune responses.
  • DNA immunization involves injecting DNA encoding antigenic proteins into animals.

Purpose of the Study:

  • To review the current knowledge on DNA immunization.
  • To summarize vectors, immune responses, and immunological mechanisms.
  • To discuss safety considerations and future applications of DNA vaccination.

Main Methods:

  • Review of existing scientific literature on DNA immunization.
  • Analysis of preclinical animal models for various diseases.
  • Examination of immune response induction, including antibody and cell-mediated immunity.

Related Experiment Videos

Main Results:

  • DNA immunization has successfully elicited protective immune responses in diverse preclinical models.
  • The method is particularly effective for inducing cytotoxic T cells.
  • A wide range of viral, bacterial, and parasitic diseases have been targeted.

Conclusions:

  • DNA immunization is a promising novel method for inducing protective immunity.
  • Further research into vectors, mechanisms, and safety is ongoing.
  • Potential applications for DNA vaccination are extensive.