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Related Experiment Videos

Molecular cloning, sequence, expression, and processing of the interleukin 16 precursor

M Baier1, N Bannert, A Werner

  • 1Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, 63225 Langen, Germany. M.Baier@em.uni-frankfurt.de

Proceedings of the National Academy of Sciences of the United States of America
|May 13, 1997
PubMed
Summary

Interleukin 16 (IL-16) is synthesized as a large precursor protein (pro-IL-16), not the previously assumed smaller form. This precursor is processed in CD8+ cells, and IL-16 expression is mainly in lymphatic tissues.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Interleukin 16 (IL-16) has known roles in T-cell activation, immune regulation, and HIV replication.
  • Inconsistencies in published IL-16 cDNA sequences suggested a precursor form (pro-IL-16).

Purpose of the Study:

  • To identify the true transcriptional start site of IL-16 mRNA.
  • To characterize the precursor form of IL-16 and its processing.

Main Methods:

  • Rapid amplification of cDNA ends (RACE) to determine the transcriptional start site.
  • Molecular cloning, sequencing, and expression of pro-IL-16 cDNA in COS-7 cells.
  • Analysis of pro-IL-16 processing in CD8+ cell lysates.

Main Results:

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  • Pro-IL-16 is likely synthesized as a 67-kDa protein encoded by a 2.6-kb transcript.
  • Recombinant pro-IL-16 is cleaved in CD8+ cell lysates, indicating a smaller bioactive form.
  • IL-16 mRNA expression is predominantly found in lymphatic tissues.

    • Conclusions:

    • The true form of IL-16 is a processed product of a larger precursor (pro-IL-16).
    • IL-16's restricted expression pattern highlights its specific immune regulatory functions.