Inhibition of melibiose transporter by amiloride in Escherichia coli

Amiloride inhibited the active transport of melibiose via the melibiose transporter in Escherichia coli. Since amiloride is known to inhibit the Na+/H+ antiporter which is essential for the establishment of an electrochemical potential of Na+ that is the driving force for melibiose transport, we tested the effect of amiloride on the activity of the melibiose transporter itself. Amiloride inhibited the melibiose counterflow. Also, inhibition of Na+ uptake induced by melibiose influx and some inhibition of H+ uptake induced by melibiose influx were observed. These results indicate that amiloride directly inhibits the melibiose transporter, perhaps by competing with Na+. It seems that the Na+ binding site and the H+ binding site in the melibiose transporter are somehow different from each other judging from the difference in the inhibition pattern of amiloride.