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Integrin beta4 is involved in apoptotic signal transduction in endothelial cells
1Sugashima Marine Biological Laboratory, School of Science, Nagoya University Toba, Mie, Japan.
Abstract:
To clarify the signal transduction in vascular endothelial cells (VEC) apoptosis induced by deprivation of FGF and serum, we investigated the function of integrin beta4 by using the monoclonal antibody (mAb) of this integrin. We added anti-beta 4 integrin mAb at the concentration of 5 microg/ml to the cells deprived of FGF and serum, apoptosis of these cells were completely inhibited 24 h after the treatment. Furthermore we plated the cells onto untreated bacterial culture plates on which the cells cannot adhere and spread in MCDB medium without FGF and serum; however, when anti-beta 4 integrin mAb was present at 5 microg/ml in the seeding medium, the cells rapidly adhered and spread. Our results first demonstrated that integrin beta4 participated in apoptotic signaling in VEC, and our findings indicate that hemidesmosome structures and keratin filament system might be important in regulation of apoptotic signaling.
Insights
Integrin beta4 prevents vascular endothelial cell (VEC) apoptosis by regulating cell adhesion and spreading. This finding highlights integrin beta4
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- Vascular endothelial cells (VECs) undergo apoptosis when deprived of fibroblast growth factor (FGF) and serum.
- The precise signal transduction pathways regulating VEC apoptosis are not fully understood.
Purpose of the Study:
- To investigate the role of integrin beta4 in VEC apoptosis induced by FGF and serum deprivation.
- To elucidate the involvement of integrin beta4 in VEC survival signaling.
Main Methods:
- Utilized a monoclonal antibody (mAb) targeting integrin beta4.
- Treated VECs with anti-beta4 integrin mAb during FGF and serum deprivation.
- Assessed cell adhesion and spreading on non-adherent surfaces in the presence of the mAb.
Main Results:
- Complete inhibition of VEC apoptosis was observed 24 hours after treatment with anti-beta4 integrin mAb.
- VECs rapidly adhered and spread on non-adherent surfaces when cultured with anti-beta4 integrin mAb.
- Integrin beta4 was demonstrated to participate in apoptotic signaling in VECs.
Conclusions:
- Integrin beta4 plays a crucial role in preventing VEC apoptosis.
- Hemidesmosome structures and the keratin filament system may be important regulators of apoptotic signaling in VECs.