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Type III collagen messenger RNA is modulated in non-compliant human bladder tissue
E P Kaplan1, J C Richier, P S Howard
1Department of Urology, University of Texas Southwestern Medical Center, Dallas 75235-9110, USA.
The Journal of Urology
|June 1, 1997
Summary
Non-compliant bladders show higher levels of type III collagen messenger RNA (mRNA). This suggests that increased collagen protein in these bladders is regulated by gene transcription.
Area of Science:
- Urology
- Molecular Biology
- Biochemistry
Background:
- Non-compliant bladders exhibit increased extracellular matrix protein, particularly type III collagen, in the muscle wall.
- Previous research indicates a correlation between histological changes and protein expression in bladder dysfunction.
Purpose of the Study:
- To investigate if elevated type III collagen messenger RNA (mRNA) levels correspond with increased protein expression in non-compliant bladders.
- To determine the transcriptional regulation of type III collagen accumulation in bladder tissue.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was employed to measure type III collagen mRNA concentrations.
- Bladder tissue samples were analyzed from a control group (n=8) with normal compliance and an experimental group (n=7) with non-compliant bladders.
Main Results:
- Type III collagen mRNA levels were significantly higher in the non-compliant bladder group (11.72 +/- 2.56 amol/mg) compared to the control group (4.26 +/- 0.74 amol/mg).
- The difference in mRNA levels between the groups was statistically significant (p = 0.027), indicating a nearly threefold increase.
Conclusions:
- Human non-compliant bladders demonstrate elevated levels of type III collagen mRNA.
- The findings support the conclusion that type III collagen protein accumulation in non-compliant bladders is, at least partly, regulated at the transcriptional level.