Correlation of retention behaviour of steroidal pharmaceuticals in polar and bonded reversed-phase liquid column...

For systematization of the correlation between the chemical structures of solutes and retention behaviour in liquid column chromatography, the retention volumes of 43 modified steroids on silica and chemically bonded reversed-phase columns were examined using various binary solvent systems. Retention parameters for the functional groups of the steroids were calculated according to Martin's additive rule. By comparing these values obtained on normal and reversed-phase columns, characteristic features of both packings with regard to solute structures and the solvent systems were elaborated.