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Related Experiment Videos

High body temperature induces micronuclei in mouse bone marrow

S Asanami1, K Shimono

  • 1Naruto Research Institute, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan.

Mutation Research
|April 24, 1997
PubMed
Summary

High body temperatures (39.5°C+) for over 30 minutes significantly increase micronuclei in mouse bone marrow cells. This suggests heat stress can cause genetic damage, potentially by disrupting cell division.

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Area of Science:

  • Toxicology
  • Genetics
  • Environmental Health

Background:

  • Elevated body temperature (hyperthermia) is a stressor that can impact cellular function.
  • The genotoxic effects of hyperthermia are not fully understood.
  • The mouse micronucleus test is a standard assay for detecting chromosomal damage.

Purpose of the Study:

  • To investigate the relationship between high body temperature and micronucleus induction in mice.
  • To determine the threshold temperature and duration for heat-induced genotoxicity.

Main Methods:

  • Male ddY mice were exposed to various temperatures (30°C, 37°C, 40°C) for different durations.
  • Bone marrow cells were collected 24 hours post-exposure.
  • Micronucleated polychromatic erythrocyte frequencies were analyzed.

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Main Results:

  • Temperatures of 37°C for 3-4 hours and 40°C for 1-2 hours significantly increased micronuclei frequencies.
  • These conditions raised mouse body temperature to approximately 40.5°C.
  • A notable percentage (25%) of induced micronuclei were relatively large.

Conclusions:

  • Body temperatures of 39.5°C or higher for more than 30 minutes induce micronuclei in mouse bone marrow.
  • Heat stress may cause genotoxicity through mechanisms like disturbance of the mitotic apparatus.