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Antigen-specific suppressor factors produced by T cell hybridomas for delayed-type hypersensitivity
European Journal of Immunology
|July 1, 1979
Summary
Researchers generated hybridoma lines that secrete factors suppressing delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC). These factors show potential for modulating immune responses, offering insights into T cell-mediated immunity.
Area of Science:
- Immunology
- Cell Biology
- Hybridoma Technology
Background:
- Delayed-type hypersensitivity (DTH) is a T cell-mediated immune response.
- Suppressor T cells play a critical role in regulating immune responses, including DTH.
Purpose of the Study:
- To generate hybridoma lines secreting factors that specifically suppress DTH.
- To characterize the activity and specificity of these secreted suppressor factors.
Main Methods:
- Fusion of AKR T lymphoma BW 5147 cells with SRBC-primed mouse spleen cells.
- Screening hybridoma supernatants for suppression of DTH induction or expression.
- Testing serum from mice bearing hybridoma lines for suppressor activity.
Main Results:
- Six hybridoma lines produced factors suppressing DTH expression.
- Four lines produced factors suppressing DTH induction, with one being antigen-specific.
- Suppressor activity was detected in serum up to a 10(-4) dilution and was effective across the H-2 barrier.
Conclusions:
- Successfully generated hybridoma lines secreting specific DTH suppressor factors.
- Identified factors capable of suppressing both induction and expression of DTH.
- Demonstrated the potential therapeutic utility of these factors in modulating T cell-mediated immunity.