Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The antigen receptor complex on cord B lymphocytes

P J Macardle1, H Weedon, M Fusco

  • 1Child Health Research Institute, Women's and Children's Hospital, North Adelaide, Australia.

Immunology
|March 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patients with cancer who will be cured and projections of complete prevalence in Italy from 2018 to 2030.

ESMO open·2024
Same author

Hypertension in childhood.

Journal of biological regulators and homeostatic agents·2020
Same author

Eculizumab treatment in patients with COVID-19: preliminary results from real life ASL Napoli 2 Nord experience.

European review for medical and pharmacological sciences·2020
Same author

White Matter Disease and Outcomes of Mechanical Thrombectomy for Acute Ischemic Stroke.

AJNR. American journal of neuroradiology·2020
Same author

Congenital renal anomalies imaging: a valuable tool for pediatricians.

Journal of biological regulators and homeostatic agents·2019
Same author

Monosymptomatic enuresis: the therapeutic weapons.

Journal of biological regulators and homeostatic agents·2019

Neonatal B cells have higher levels of IgM, a key antibody, on their surface. This may help them respond to antigens, but further research is needed on their activation.

Area of Science:

  • Immunology
  • Neonatal immunology
  • B-cell biology

Background:

  • The neonatal immune system has a limited antigen response, primarily producing low-affinity IgM antibodies.
  • B-cell antigen receptor complex composition differs between neonatal and adult cells.

Purpose of the Study:

  • To compare the B-cell antigen receptor complex components in neonatal versus adult B cells.
  • To investigate the functional implications of these differences on B-cell activation.

Main Methods:

  • Flow cytometry was used to quantify cell surface marker expression (IgM, IgD, CD79, CD19, CD21, CD22, CD81, CD32).
  • In vitro experiments assessed neonatal B-cell responses to interleukin-4 (IL-4) and anti-IgM activation.

Main Results:

Related Experiment Videos

  • Neonatal B cells exhibit significantly elevated membrane IgM and CD79 levels compared to adult B cells.
  • Reduced levels of the inhibitory receptor CD32 were observed on neonatal B cells.
  • While IL-4 further increased IgM levels, in vitro activation assays showed no significant functional differences in response to anti-IgM.

Conclusions:

  • Elevated membrane IgM on neonatal B cells may enhance sensitivity to low antigen concentrations.
  • Despite molecular differences, neonatal B cells do not show impaired activation via the B-cell receptor pathway in vitro.
  • Further studies are needed to fully elucidate the functional consequences of these neonatal B-cell characteristics.