Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional, morphological, and phenotypical differences between rat alveolar and interstitial macrophages

A Johansson1, M Lundborg, C M Sköld

  • 1Division of Inhalation Toxicology, Institute of Environmental Medicine, Karolinska Institute, Stockholm, Sweden.

American Journal of Respiratory Cell and Molecular Biology
|May 1, 1997
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal and cross-sectional analysis of perfluoroalkyl substances and kidney function.

Journal of exposure science & environmental epidemiology·2025
Same author

Variability of Normal Pressure Hydrocephalus Imaging Biomarkers with Respect to Section Plane Angulation: How Wrong a Radiologist Can Be?

AJNR. American journal of neuroradiology·2021
Same author

Diagnosing Carotid Near-Occlusion with Phase-Contrast MRI.

AJNR. American journal of neuroradiology·2021
Same author

Changes in lung immune cells related to clinical outcome during treatment with infliximab for sarcoidosis.

Clinical and experimental immunology·2020
Same author

Can pulsatile CSF flow across the cerebral aqueduct cause ventriculomegaly? A prospective study of patients with communicating hydrocephalus.

Fluids and barriers of the CNS·2019
Same author

Risk factors for pancreatitis following endoscopic retrograde cholangiopancreatography.

BJS open·2019

Interstitial macrophages (IM) and alveolar macrophages (AM) in rat lungs exhibit distinct phenotypes. IM are smaller, less phagocytic, and possess unique surface receptors, indicating significant immunocompetence.

Area of Science:

  • Pulmonary immunology
  • Cell biology
  • Macrophage biology

Background:

  • Alveolar macrophages (AM) and interstitial macrophages (IM) are key immune cells in the lungs.
  • Their distinct roles and characteristics are not fully understood.

Purpose of the Study:

  • To comprehensively characterize rat lung AM and IM.
  • To compare their morphology, phagocytosis, adhesion, and surface receptor phenotype.

Main Methods:

  • Lung lavage for AM isolation.
  • Enzymatic digestion (DNAse, collagenase) for IM isolation.
  • Morphological analysis, phagocytosis assays, particle attachment studies, and surface receptor expression analysis (OX-1, CD71, OX-42, MHC class Ia, CD54).

Main Results:

Related Experiment Videos

  • IM are smaller than AM with a higher nuclear-to-cytoplasm ratio.
  • IM exhibit lower particle attachment but similar ingestion capacity compared to AM.
  • AM show higher adherence to vitronectin and fibronectin.
  • Significant differences in the expression of five surface receptors were observed between AM and IM.
  • IM express higher levels of MHC class Ia and CD54, suggesting enhanced antigen presentation capabilities.

Conclusions:

  • Rat lung AM and IM display profound phenotypic differences.
  • IM are highly immunocompetent cells, not merely precursors to AM.
  • These distinct phenotypes suggest specialized functions within the lung immune environment.