[Validity of a liquid chromatographic (HPLC) method for the determination of ticlopidine in human plasma]

A simple high-performance liquid chromatographic method for determination of ticlopidine in human plasma using UV detection was developed. The separation of the investigated compound and internal standard was achieved on "BST Rutin" 10, C18 BD column with a 0.01 M, (pH 4) potassium dihydrogen phosphate bufferacetonitrile-methanol (20:40:40 v/v) mobile phase. The detection was performed at 215 nm. The compounds were isolated from plasma by Bond Elut C18 solid-phase extraction, the mean absolute recovery was 84.9%. The limit of quantitation was 10 ng/ml, the limit of detection was 5 ng/ml. The assay has been validated with respect to accuracy, precision and system suitability. All validated parameters were found to be within the necessary limits. The developed analytical method for ticlopidine was found to be suitable for application in pharmacokinetic studies and human drug monitoring.