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An expression vector encoding a lacZ reporter gene facilitates identification of stable, high-producing CHO cell
T H Grossman1, J E Godoy, E S Kawasaki
1Department of Molecular Biology, Procept. Inc., Cambridge, Massachusetts, USA.
Plasmid
|January 1, 1997
Abstract:
We describe a vector and a streamlined procedure for isolating high-producing stable mammalian cell transfectants. The vector encodes the Escherichia coli lacZ gene as a reporter. We show that levels of beta-galactosidase activity, assayed in situ in clonal isolates, can be used to identify clones producing high levels of the protein of interest (in this case, soluble human CD4 protein).