An expression vector encoding a lacZ reporter gene facilitates identification of stable, high-producing CHO cell clones
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Updated: Nov 1, 2026

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
Published on: June 19, 2013
T H Grossman1, J E Godoy, E S Kawasaki
1Department of Molecular Biology, Procept. Inc., Cambridge, Massachusetts, USA.
Abstract:
We describe a vector and a streamlined procedure for isolating high-producing stable mammalian cell transfectants. The vector encodes the Escherichia coli lacZ gene as a reporter. We show that levels of beta-galactosidase activity, assayed in situ in clonal isolates, can be used to identify clones producing high levels of the protein of interest (in this case, soluble human CD4 protein).
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