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Updated: Sep 12, 2026

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
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Published on: December 14, 2016

Do human B and null lymphocytes form a single immunoglobulin-bearing population?

D G Haegert, R R Coombs

    Lancet (London, England)
    |November 17, 1979
    PubMed

    Human "null" lymphocytes have been recognised because they lack T-lymphocyte membrane markers and the surface membrane immunoglobulin (smIg), detectable by direct immunofluorescence, which conventionally characterises B lymphocytes. But almost all null lymphocytes express smIg determinants which are demonstrable by the direct antiglobulin and mixed antiglobulin rosette tests. It is argued that the definition of a B lymphocyte should be extended to include the null cells which react in these tests. If the heterogeneity of the B-lymphocyte population is more widely recognized, the precise nature of human natural and antibody-dependent killer cells may become clearer.

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