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Alternative Methods for the Detection of Superoxide Anion Generation in Platelets06:35

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets

The generation of superoxide anion is essential for the stimulation of platelets and, if dysregulated, critical for thrombotic diseases. Here, we present three protocols for the selective detection of superoxide anions and the study of redox-dependent platelet...
1.1K
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Mitochondria contain several flavin-dependent enzymes that can produce reactive oxygen species (ROS). Monitoring ROS release from individual sites in mitochondria is challenging due to unwanted side reactions. We present an easy, inexpensive method for direct assessment of native rates for ROS release using purified flavoenzymes and microplate...
10.5K
Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds04:58

Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds

This article provides a detailed and visual description of a methodology for collecting and measuring biochemical inflammatory and nociceptive mediators at the surgical wound site following cesarean delivery. This human bioassay has been used to determine correlations between wound and serum cytokine concentrations and drug-mediated changes in wound cytokines, chemokines and...
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Measuring the 50% Haemolytic Complement (CH50) Activity of Serum08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of...
38.9K
Determining the Reactivity and Titre of Serum using a Haemagglutination Assay05:59

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Haemagglutination is a form of agglutination where antibodies bind to red blood cells. Red blood cells are both readily available and the results are readily observable using the naked eye. This video demonstrates the steps involved in a haemagglutination assay, interpreting the results and determining the...
21.5K
Determining the Role of Vitronectin in Bacterial Resistance to Bactericidal Activity of Human Serum02:55

Determining the Role of Vitronectin in Bacterial Resistance to Bactericidal Activity of Human Serum

This video demonstrates the role of vitronectin, a human serum glycoprotein, on bacterial resistance. Antibodies in the serum interact with bacterial surface proteins, activating complement proteins and forming the membrane attack complex, causing bacterial cell lysis. Vitronectin binding on bacterial membrane protein prevents the membrane attack complex formation, resulting in bacterial...
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