Amperometric immunosensors based on rigid conducting immunocomposites

Area of Science:

• Biomaterials Science
• Analytical Chemistry
• Immunosensor Technology

Background:

• Development of sensitive and reusable biosensing platforms is crucial for diagnostics.
• Existing immunosensors often face limitations in regeneration and material stability.
• Biocomposite materials offer unique properties for integrating biological recognition elements and signal transduction.

Purpose of the Study:

• To construct and characterize novel, polishable immunosensors based on rigid biocomposite materials.
• To develop a competitive binding assay for the quantification of rabbit IgG (RIgG).
• To evaluate the analytical performance and reusability of the developed immunosensor.

Main Methods:

• Fabrication of biocomposite materials incorporating graphite powder, rabbit IgG, and methacrylate or epoxy resins.
• Development of a competitive immunoassay using goat anti-rabbit IgG labeled with alkaline phosphatase.
• Amperometric detection of phenol produced from phenyl phosphate substrate at 800 mV (vs Ag/AgCl).
• Regeneration of the immunosensor surface through polishing.

Main Results:

• Successful construction of novel biocomposite-based immunosensors.
• Demonstration of a competitive binding assay for RIgG detection.
• Amperometric detection of enzymatic product indicating successful binding events.
• Achieved sensor regeneration via polishing, enabling multiple assay cycles.

Conclusions:

• The developed biocomposite immunosensors are polishable and reusable, offering a stable platform for immunoassays.
• This novel material integrates biological recognition and signal transduction, simplifying sensor design.
• The immunosensor shows potential for sensitive and repeatable determination of rabbit IgG in samples.