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In-vitro activity of lansoprazole against Helicobacter pylori

N Figura1, J E Crabtree, M Dattilo

  • 1Institute of Internal Medicine, University of Siena, Italy. figura@unisi.it

The Journal of Antimicrobial Chemotherapy
|May 1, 1997
PubMed

Insights

Lansoprazole effectively kills Helicobacter pylori, with its sulfenamide form showing faster action. This proton pump inhibitor is a promising addition to antibiotic treatments for H. pylori infections.

Area of Science:

  • Microbiology
  • Pharmacology

Background:

  • Lansoprazole, a proton pump inhibitor, demonstrates in vitro activity against Helicobacter pylori.
  • The precise mechanism of lansoprazole's antimicrobial effect requires further elucidation.

Purpose of the Study:

  • To investigate the antimicrobial mechanism of lansoprazole against Helicobacter pylori.
  • To compare the activity of lansoprazole and its sulfenamide derivative against H. pylori strains.

Main Methods:

  • Determining Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentrations (MBCs) for lansoprazole and its sulfenamide against 19 H. pylori strains.
  • Assessing the rate of bacterial killing and evaluating the effect on toxin-induced vacuolization in HeLa cells.

Main Results:

  • Lansoprazole and its sulfenamide exhibited similar MIC90 (2.5 mg/L) and MBC90 (10 mg/L) values against H. pylori.
  • Cytotoxic and non-cytotoxic H. pylori strains showed comparable susceptibility.
  • The sulfenamide derivative demonstrated more rapid bactericidal activity.
  • Lansoprazole did not inhibit toxin-induced vacuolization, indicating its anti-H. pylori activity is independent of v-ATPase inhibition.

Conclusions:

  • Sulfenamide formation in acidic gastric environments likely enhances lansoprazole's in vivo bactericidal activity.
  • Lansoprazole, potentially in combination with antibiotics, is a viable treatment option for H. pylori infections, irrespective of bacterial cytotoxicity.

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