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Updated: Jul 20, 2026

Protocol for Long Duration Whole Body Hyperthermia in Mice
Published on: August 25, 2012
Efficacy of hyperthermia and polyunsaturated fatty acids on experimental carcinoma
S Kokura1, T Yoshikawa, T Kaneko
1First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kamigyo-ku, Japan.
Abstract:
We investigated the efficacy of hyperthermia and gamma-linolenic acid on experimental carcinoma. This study focused on polyunsaturated fatty acids that are substrates for free radical reactions. Oleic acid, linolenic acid, alpha-linolenic acid, or gamma-linolenic acid was injected into the arteries feeding AH109A carcinoma implanted into rat hind limbs. Among these, gamma-linolenic acid had the greatest effect on tumor tissue lipid peroxidation and demonstrated an antitumor effect. Consequently, gamma-linolenic acid injection into the feeding artery of a tumor was performed immediately prior to hyperthermia. This combination therapy induced a high level of lipid peroxidation in tumor tissue and a significant antitumor effect. Hyperthermia combined with gamma-linolenic acid produces free radical reactions by increasing the radical reaction substrate and may be an effective anticancer modality.
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