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Ozone stress modulates amine oxidase and lipoxygenase expression in lentil (Lens culinaris) seedlings
M Maccarrone1, G A Veldink, F G Vliegenthart
1Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Italy.
Abstract:
The effect of ozone stress on polyamine metabolism and membrane lipid peroxidation in lentil seedlings through the amine oxidase and lipoxygenase activity and expression has been investigated. Ozone is shown to control the expression of these enzymes at the transcriptional level, down-regulating the amine oxidase gene and up-regulating the lipoxygenase gene. The decrease of amine oxidase activity correlated with the increase of putrescine concentration in the ozone-treated plantlets, whereas the increase of lipoxygenase activity was paralleled by enhanced membrane lipid peroxidation. Finally, polyamines are shown to inhibit lipoxygenase activity in lentils.