Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered monocyte calcium dynamics in sepsis

H Shin1, R Charlton, D L Mollitt

  • 1Department of Surgery, University of Florida, Jacksonville 32209, USA.

The Journal of Trauma
|May 1, 1997
PubMed
Summary

Sepsis significantly alters monocyte calcium levels and mobilization. A serum factor, not endotoxin, appears responsible for these calcium dynamics changes in septic patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust Statistical Approaches to Understanding the Causal Effect of Air Pollution Mixtures.

Research report (Health Effects Institute)·2026
Same author

Photon-counting detector CT (PCD-CT) generated iodine maps to characterize parenchymal lung disease: A feasibility study.

European journal of radiology·2024
Same author

Evaluation of force pain thresholds to ensure collision safety in worker-robot collaborative operations.

Frontiers in robotics and AI·2024
Same author

Detection of novel drug-adverse drug reaction signals in rheumatoid arthritis and ankylosing spondylitis: analysis of Korean real-world biologics registry data.

Scientific reports·2024
Same author

An extremely energetic cosmic ray observed by a surface detector array.

Science (New York, N.Y.)·2023
Same author

Phase I dose escalation and expansion study of golidocitinib, a highly selective JAK1 inhibitor, in relapsed or refractory peripheral T-cell lymphomas.

Annals of oncology : official journal of the European Society for Medical Oncology·2023

Area of Science:

  • Immunology
  • Cellular Biology
  • Biochemistry

Background:

  • Monocytes play a crucial role in the immune response.
  • Calcium ions are critical for cellular signaling and function, including immune cell activation.
  • Sepsis, a life-threatening condition, involves a dysregulated immune response.

Purpose of the Study:

  • To investigate alterations in monocyte calcium concentration and mobilization in surgical patients with sepsis.
  • To compare these calcium dynamics between septic and healthy individuals.

Main Methods:

  • Monocytes were isolated from septic patients and healthy volunteers.
  • Intracellular calcium concentration ([Ca2+]i) was measured using FURA-2 and fluorescent spectrophotometry.
  • Cells were stimulated with concanavalin A, and experiments included incubation with septic serum, normal serum, and endotoxin.

Main Results:

  • Septic monocytes exhibited lower resting calcium concentrations compared to normal monocytes.
  • While both groups showed calcium mobilization upon stimulation, septic monocytes reached lower final calcium levels.
  • Normal monocytes incubated with septic serum showed similar alterations, suggesting a serum-mediated effect distinct from endotoxin.

Conclusions:

  • Sepsis is associated with significant changes in both resting and stimulated monocyte calcium dynamics.
  • A serum factor, independent of endotoxin, appears to modulate these calcium alterations in sepsis.

Related Experiment Videos