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The transcriptional co-activator p/CIP binds CBP and mediates nuclear-receptor function
J Torchia1, D W Rose, J Inostroza
1Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093-0648, USA.
Nature
|June 12, 1997
Summary
A newly discovered factor, p/CIP, works with CBP and p300 to activate DNA transcription. It is essential for nuclear receptor activity and gene activation, highlighting its role in cellular signaling pathways.
Area of Science:
- Molecular Biology
- Gene Regulation
- Protein Interactions
Background:
- CBP and p300 are conserved proteins crucial for DNA transcription activation.
- Nuclear receptors regulate gene expression, often requiring co-activator proteins.
Purpose of the Study:
- To identify and characterize novel factors involved in transcriptional regulation.
- To elucidate the role of p/CIP and NCoA-1 in nuclear receptor-mediated gene activation.
Main Methods:
- Co-immunoprecipitation assays to detect protein complexes.
- Analysis of transcriptional activity in response to nuclear receptor ligands.
- Identification of conserved protein domains involved in gene activation.
Main Results:
- A novel factor, p/CIP, was identified and found to form a complex with CBP.
- p/CIP is essential for the transcriptional activity of nuclear receptors and other CBP/p300-dependent factors.
- The nuclear receptor co-activator NCoA-1 is also critical for ligand-dependent gene activation.
- p/CIP, NCoA-1, and CBP share leucine-rich motifs required for receptor-specific gene activation and signal transduction pathway modulation.
Conclusions:
- p/CIP is a key co-activator for nuclear receptors and CBP/p300-dependent transcription.
- The identified leucine-rich motifs mediate specific gene activation mechanisms and pathway inhibition.
- These findings expand our understanding of the molecular mechanisms governing gene transcription.