Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MultiCoil: a program for predicting two- and three-stranded coiled coils

E Wolf1, P S Kim, B Berger

  • 1Howard Hughes Medical Institute, Whitehead Institute, MIT, Cambridge, Massachusetts 02142, USA.

Protein Science : a Publication of the Protein Society
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Predictors of fatigue improvement in multimodal, multimodal-aerobic and aerobic exercise intervention studies in breast cancer survivors with cancer-related fatigue.

Scientific reports·2025
Same author

Predictors of fatigue improvement in multimodal, multimodal-aerobic and aerobic exercise intervention studies in breast cancer survivors with cancer-related fatigue.

Scientific reports·2025
Same author

Inadvertent intrathecal application of vindesine and its neurological outcome: case report and systematic review of the literature.

Brain & spine·2025
Same author

[Teaching otorhinolaryngology in times of COVID-19: to what extent can digital formats replace face-to-face teaching?]

HNO·2022
Same author

Evaluation of commercial composts and potting mixes and their ability to support arbuscular mycorrhizal fungi with maize (Zea mays) as host plant.

Waste management (New York, N.Y.)·2021
Same author

Using High-Throughput Phenotyping to Explore Growth Responses to Mycorrhizal Fungi and Zinc in Three Plant Species.

Plant phenomics (Washington, D.C.)·2020
Same journal

Macromolecular crowding inhibits degradation of alpha-synuclein amyloid fibrils induced by cathepsins and MMP9.

Protein science : a publication of the Protein Society·2026
Same journal

Sequence-encoded differences in the conformational ensembles of CITED transcriptional activation domains impact coactivator binding.

Protein science : a publication of the Protein Society·2026
Same journal

The phospholipid biosynthesis enzyme PlsB contains three distinct domains for membrane association, lysophosphatidic acid synthesis, and dimerization.

Protein science : a publication of the Protein Society·2026
Same journal

Structural basis of ligand selectivity in FAD/NAD(P)H-dependent dehydrogenases: insights from trypanothione reductase and type II NADH dehydrogenase.

Protein science : a publication of the Protein Society·2026
Same journal

Achieving protease substrate-specific inhibition by mAb dual functional selections.

Protein science : a publication of the Protein Society·2026
Same journal

How important are quantum mechanical effects in controlling biological functions: Enzymes, electron transfer and bird navigation.

Protein science : a publication of the Protein Society·2026
See all related articles

The MultiCoil program enhances coiled-coil identification by distinguishing between dimeric and trimeric structures. This new approach improves predictions for protein structures, aiding in understanding protein function.

Area of Science:

  • Proteomics
  • Bioinformatics
  • Structural Biology

Background:

  • Coiled coils are protein structural motifs crucial for protein-protein interactions.
  • Existing prediction tools primarily focus on dimeric coiled coils, limiting analysis of other oligomeric states.

Purpose of the Study:

  • To introduce the MultiCoil program for identifying and distinguishing between dimeric and trimeric coiled coils.
  • To extend coiled-coil prediction capabilities beyond two-stranded structures.

Main Methods:

  • Implementation of a multidimensional scoring approach within the MultiCoil program.
  • Utilizing a comprehensive database of three-stranded coiled coils (6,319 residues) and a two-stranded coiled-coil database.
  • Extending the PairCoil program to accommodate three-stranded coiled-coil identification.

Related Experiment Videos

Main Results:

  • MultiCoil successfully identifies coiled coils missed by two-stranded prediction programs.
  • The program accurately classifies the oligomerization states of known dimeric and trimeric coiled coils.
  • Estimates suggest 0.9% of protein residues form three-stranded and 1.5% form two-stranded coiled coils.

Conclusions:

  • The MultiCoil program offers a significant advancement in analyzing coiled-coil structures.
  • The findings provide insights into the prevalence and structural features of different coiled-coil oligomerization states.
  • MultiCoil is publicly available for researchers to analyze protein structures.