Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of a quantitative sampling method for periapical exudates from human root canals

H Shimauchi1, Y Miki, S Takayama

  • 1Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.

Journal of Endodontics
|November 1, 1996
PubMed
Summary

A new quantitative method effectively collects periapical exudates using endodontic paper points. This technique allows for the measurement of interleukin-1 beta (IL-1β) in apical periodontitis, aiding in the analysis of immunological changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Coagulation and fibrinolysis in pregnancy].

Rinsho byori. The Japanese journal of clinical pathology·1992
Same author

[Recent progress and clinical significance of complement system].

Ryumachi. [Rheumatism]·1992
Same author

A new nylon oligomer degradation gene (nylC) on plasmid pOAD2 from a Flavobacterium sp.

Journal of bacteriology·1992
Same author

Immunohistochemical localization of 17 alpha-hydroxylase/C17-20 lyase and aromatase cytochrome P-450 in the human ovary during the menstrual cycle.

The Journal of endocrinology·1992
Same author

Modulation of transient type K channel cloned from rat heart.

Biochemical and biophysical research communications·1992
Same author

Site-directed mutagenesis at aspartate and glutamate residues of xylanase from Bacillus pumilus.

The Biochemical journal·1992

Area of Science:

  • Endodontics
  • Immunology
  • Biomaterials

Background:

  • Apical periodontitis involves complex immunological changes.
  • Accurate collection of periapical exudates is crucial for analysis.
  • Existing methods may lack quantitative precision.

Purpose of the Study:

  • To describe a novel quantitative method for collecting periapical exudates on endodontic paper points.
  • To validate the relationship between fluid volume and paper point characteristics.
  • To assess the utility of this method for measuring interleukin-1 beta (IL-1β) activity in clinical samples.

Main Methods:

  • Development of a quantitative paper point collection technique for periapical exudates.
  • Pilot study to establish relationships between fluid volume, wetted length, and IL-1β activity.

Related Experiment Videos

  • Application of the method to collect exudates from 29 root canals with apical periodontitis.
  • Measurement of IL-1β concentrations in collected periapical exudates.

    • Main Results:

    • A highly significant curvilinear relationship was found between fluid volume and wetted paper point length (p < 0.0001).
    • A significant positive linear relationship was observed for eluted and absorbed IL-1β activities.
    • Recovered periapical exudates ranged from 0.15 to 26.7 microliters.
    • IL-1β concentrations varied from 0.1 to 179.5 ng/ml.

    Conclusions:

    • The described quantitative method is effective for collecting periapical exudates.
    • This technique enables reliable measurement of IL-1β in clinical specimens.
    • The method is valuable for analyzing immunological changes in periapical lesions.