Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The steric trigger in rhodopsin activation

T Shieh1, M Han, T P Sakmar

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

Journal of Molecular Biology
|June 13, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipid metabolism of hepatocyte-like cells supports intestinal tumor growth in Drosophila.

Nature communications·2026
Same author

In reply to the comment on "Does preoperative joint space affect accuracy in splint-based Le Fort I osteotomy?"

International journal of oral and maxillofacial surgery·2026
Same author

[Mediation effect analysis on aromatic hydrocarbon receptors and DNA oxidative damage with embryonic arrest caused by polycyclic aromatic hydrocarbon exposure].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2026
Same author

[Protective effect of knock-down the expression of <i>Blimp1</i> gene on early liver injury in CCl<sub>4</sub>-induced mouse model of liver fibrosis].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

Lipid metabolism of hepatocyte-like cells supports intestinal tumor growth by promoting tracheogenesis.

bioRxiv : the preprint server for biology·2025
Same author

Automatic pituitary adenoma segmentation and identification of cavernous sinus invasion via multitask learning.

Clinical radiology·2024
Same journal

Clinical inflammasome biomarkers: Progress and prospects.

Journal of molecular biology·2026
Same journal

Biologically Relevant, Cationic Residues in Human Rhinovirus Stabilize Capsid-Bound RNA Duplexes, and Restrict Capsid Flexibility.

Journal of molecular biology·2026
Same journal

Cryo-EM structures of phage T4 infection intermediate.

Journal of molecular biology·2026
Same journal

A classic fold with a twist: Structural architecture of Dhillonvirus phage Bas18.

Journal of molecular biology·2026
Same journal

Tesorai Search: cloud-based database search engine boosts identifications for mass spectrometry proteomics with a pretrained peptide-spectrum deep-learning model.

Journal of molecular biology·2026
Same journal

Characterization of diverse functions of NRF1 nuclear localization sequence.

Journal of molecular biology·2026
See all related articles

Rhodopsin

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Vision Science

Background:

  • Rhodopsin, a G protein-coupled receptor, mediates dim light vision.
  • Its ligand, 11-cis retinal, covalently binds and changes from antagonist to agonist upon light absorption.
  • Structural homology exists with other G protein-coupled receptors.

Purpose of the Study:

  • To elucidate the activation mechanism of rhodopsin.
  • To understand the role of retinal isomerization in receptor activation.
  • To investigate the structural basis of retinal-binding site selectivity.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy of rhodopsin and bathorhodopsin.
  • Computational docking of retinal chromophores into protein models.

Related Experiment Videos

  • Site-directed mutagenesis studies of the retinal-binding site.

    • Main Results:

    • Structural constraints from NMR enabled chromophore docking into a low-resolution rhodopsin model.
    • A mechanism for light-induced rhodopsin activation involving retinal isomerization was proposed.
    • Mutagenesis revealed modifications to favor all-trans retinal binding.
    • A direct steric interaction between retinal and transmembrane helix 3 (Gly121 region) was identified.

    Conclusions:

    • Retinal isomerization and steric interactions drive rhodopsin activation.
    • Understanding rhodopsin structure-function provides insights into G protein-coupled receptor mechanisms.
    • Mutagenesis offers a route to engineer chromophore selectivity in visual pigments.