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Amperometric detection of organic thiols at a tungsten wire electrode following their separation by liquid
A Hidayat1, D B Hibbert, P W Alexander
1Department of Analytical Chemistry, University of New South Wales, Sydney, Australia.
Abstract:
The amperometric detection of thiols following their separation by reversed-phase chromatography and reaction into a post-column mercury carrier stream is shown to be a sensitive method when using a tungsten wire sensor as the working electrode in a three-electrode flow cell. Five organic thiols (cysteine, homocysteine, reduced glutathione, D,L-penicillamine and 2-mercaptopropionic acid) and thiourea could be separated within approx. 18 min. The analytical performance is comparable, and stability superior, to chemically modified electrodes previously reported.