Optimizing multiplex and LA-PCR with betaine
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1National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA. pnh@ncifcrf.gov
Trends in Biochemical Sciences
|June 1, 1997
Abstract:
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and 'long and accurate' polymerase chain reaction (LA-PCR). For details on how to partake in the newsgroup, see the accompanying box.
