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[A human bone marrow cell line (B3HM) inducing leukemia in mouse]
1Institute of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin.
Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|March 1, 1996
Summary
A novel human cell line, B3HM, was established from bone marrow mononuclear cells. These cells exhibit characteristics of both B cells and granulocytes and can induce leukemia in mice.
Area of Science:
- Hematology
- Oncology
- Cell Biology
Context:
- Establishment of novel human cell lines is crucial for understanding disease mechanisms.
- Bone marrow mononuclear cells (BMMCs) are a source of hematopoietic stem cells.
- Cancer patient-derived stromal cells can influence BMMC behavior.
Purpose:
- To establish and characterize a novel human cell line from BMMCs.
- To investigate the potential of the established cell line to induce leukemia.
- To analyze the surface markers and growth characteristics of the new cell line.
Summary:
- Human bone marrow mononuclear cells were cultured on esophageal cancer stromal cells, leading to the establishment of the B3HM cell line.
- B3HM cells demonstrated continuous growth, anchorage independence, and expressed both B cell (CD19+, CD20+) and myeloid (CD13+, CD15+) markers.
- Inoculation of B3HM cells or homogenate induced transplantable leukemia in nude and inbred mice, with leukemic cells being L3T4 positive.
Impact:
- The B3HM cell line provides a new model for studying B cell and granulocyte development.
- This cell line can be utilized to investigate leukemia pathogenesis and potential therapeutic targets.
- The induced mouse leukemia model offers a platform for preclinical research in hematologic malignancies.