Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antioxidative constituents in Heterotheca inuloides

H Haraguchi1, H Ishikawa, Y Sanchez

  • 1Faculty of Engineering, Fukuyama University, Japan.

Bioorganic & Medicinal Chemistry
|May 1, 1997
PubMed
Summary

Mexican "arnica" (Heterotheca inuloides) contains sesquiterpenoids and flavonoids that act as potent antioxidants. These compounds protect against oxidative stress by scavenging radicals and inhibiting lipid peroxidation in different cellular compartments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for the K_{L}→π^{0}νν[over ¯] Decay at the J-PARC KOTO Experiment.

Physical review letters·2025
Same author

Study of the K_{L}→π^{0}νν[over ¯] Decay at the J-PARC KOTO Experiment.

Physical review letters·2021
Same author

Search for K_{L}→π^{0}νν[over ¯] and K_{L}→π^{0}X^{0} Decays at the J-PARC KOTO Experiment.

Physical review letters·2019
Same author

Study of cobalt(III) complexes by atomic absorption spectrometry. Effect of ligands and distribution of atomic cobalt in the flame.

Analytical chemistry·2012
Same author

Counterionic Detection by ICP-AES for Determination of Inorganic Anions in Water Elution Ion Chromatography Using a Zwitterionic Stationary Phase.

Analytical chemistry·2011
Same author

Steady-state atomic fluorescence radiance expressions for continuum excitation: errata.

Applied optics·2010

Area of Science:

  • Phytochemistry
  • Biochemistry
  • Pharmacology

Background:

  • Heterotheca inuloides, a Mexican medicinal plant known as "arnica", is traditionally used for its therapeutic properties.
  • Oxidative stress is implicated in various diseases, necessitating the identification of novel antioxidant compounds.
  • Sesquiterpenoids and flavonoids are classes of natural products with known biological activities.

Purpose of the Study:

  • To evaluate the antioxidant potential of sesquiterpenoids and flavonoids isolated from Heterotheca inuloides.
  • To investigate the specific mechanisms of antioxidant action of these compounds in different cellular models.
  • To determine the contribution of these compounds to the overall protective effects of the plant.

Main Methods:

  • Isolation and identification of sesquiterpenoids (7-hydroxy-3,4-dihydrocadalin, 7-hydroxycadalin) and flavonoids (quercetin, kaempferol, and their glycosides) from H. inuloides.

Related Experiment Videos

  • Assessment of radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.
  • Evaluation of inhibition of microsomal and mitochondrial lipid peroxidation induced by Fe(III)-ADP/NADPH and Fe(III)-ADP/NADH, respectively.
  • Measurement of superoxide anion scavenging activity in microsomes and protection of mitochondrial enzyme activity.

    • Main Results:

    • Isolated sesquiterpenoids and flavonoids exhibited potent scavenging activity against DPPH radicals.
    • Both compound classes inhibited microsomal lipid peroxidation, but only flavonoids scavenged superoxide anions in microsomes.
    • Sesquiterpenoids uniquely inhibited mitochondrial lipid peroxidation and protected mitochondrial enzyme activity against oxidative stress.
    • The study identified distinct antioxidant roles for sesquiterpenoids and flavonoids within H. inuloides.

    Conclusions:

    • Heterotheca inuloides contains at least two distinct types of antioxidants: sesquiterpenoids and flavonoids.
    • These compounds contribute to the plant's protective effects against various oxidative stresses through different mechanisms.
    • The findings support the traditional medicinal use of "arnica" and highlight its potential as a source of natural antioxidants.