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Lysosomal enzyme release during cardiopulmonary bypass
Anaesthesia
|September 1, 1977
Summary
Lysosomal enzyme release occurs during cardiopulmonary bypass. N-acetyl beta-glucosaminidase in plasma increases with bypass duration, suggesting it's a better marker of bypass-induced tissue damage.
Area of Science:
- Biochemistry
- Cardiovascular Surgery
- Cell Biology
Background:
- Lysosomal enzyme release is observed during cardiopulmonary bypass (CPB).
- The specific tissues responsible for this enzyme release during CPB remain unidentified.
- Existing markers for tissue damage during CPB may not fully distinguish between bypass effects and surgical trauma.
Purpose of the Study:
- To identify the source of lysosomal enzyme release during CPB.
- To evaluate N-acetyl beta-glucosaminidase as a specific marker for CPB-induced tissue damage.
- To compare the efficacy of N-acetyl beta-glucosaminidase with beta-glucuronidase in reflecting CPB-related tissue injury.
Main Methods:
- Analysis of plasma lysosomal enzyme activity in patients undergoing CPB.
- Measurement of N-acetyl beta-glucosaminidase and beta-glucuronidase levels.
- Correlation of enzyme activity with the duration of cardiopulmonary bypass.
Main Results:
- Plasma N-acetyl beta-glucosaminidase activity was found to increase during CPB.
- The elevation in N-acetyl beta-glucosaminidase activity correlated directly with the duration of CPB.
- This suggests a link between CPB duration and lysosomal enzyme release.
Conclusions:
- N-acetyl beta-glucosaminidase may serve as a more accurate indicator of tissue damage specifically caused by CPB.
- This enzyme's activity can help differentiate CPB-induced damage from general surgical trauma.
- Further research is warranted to pinpoint the exact tissues of origin for these enzymes during CPB.