Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Correlation between substratum roughness and wettability, cell adhesion, and cell migration

M Lampin1, Warocquier-Clérout, C Legris

  • 1Laboratoire de Biologie Cellulaire Expérimentale, Université de Technologie de Compiègne, France.

Journal of Biomedical Materials Research
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Observation of Electron Scattering from Online-Produced Radioactive Target.

Physical review letters·2023
Same author

[Is self-confidence a factor for successful breastfeeding?].

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2015
Same author

Three-dimensional pore-scale modelling of dentinal infiltration.

Computer methods in biomechanics and biomedical engineering·2012
Same author

Self-etching increases matrix metalloproteinase expression in the dentin-pulp complex.

Journal of dental research·2009
Same author

Bond strengths of nine current dentine adhesive systems to primary and permanent teeth.

Journal of oral rehabilitation·2005
Same author

Influence of drying and HEMA treatment on dentin wettability.

Dental materials : official publication of the Academy of Dental Materials·2002

Slight roughness on poly (methyl methacrylate) (PMMA) enhances cell adhesion and spreading for chick embryo explants. Surface protein adsorption negates these effects, highlighting the importance of surface properties in biocompatibility.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Surface Chemistry

Background:

  • Poly (methyl methacrylate) (PMMA) is a common biomaterial.
  • Surface properties of biomaterials influence cell behavior.
  • Understanding cell response to surface topography is crucial for biocompatibility.

Purpose of the Study:

  • To investigate cell adhesion and spreading of chick embryo vascular and corneal explants on PMMA with varying roughness.
  • To analyze the specificity of cell response to substratum surface properties.
  • To correlate biocompatibility with substratum surface state.

Main Methods:

  • PMMA surfaces were modified by sand-blasting to create different roughness degrees.
  • Surface free energy (SFE) components and contact angles were measured.

Related Experiment Videos

  • Protein adsorption from culture medium onto PMMA surfaces was assessed.
  • Organotypic culture assays were used to measure cell migration and adhesion.

    • Main Results:

    • Slight surface roughness increased the apolar component of SFE and decreased the basic component.
    • Increased roughness led to the disappearance of acido-basic properties.
    • Protein adsorption from culture medium eliminated the effect of roughness and restored hydrophilic properties.
    • Slight roughness significantly enhanced cell migration area and cell adhesion potential, correlating with surface hydrophobicity.

    Conclusions:

    • Cellular adhesion and migration are significantly influenced by the surface roughness and hydrophobicity of PMMA.
    • The biological environment, through protein adsorption, can modify surface properties and impact cell response.
    • Tailoring PMMA surface topography and energy is a promising strategy for improving biomaterial biocompatibility.