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Midkine and secondary neurulation

M Griffith1

  • 1Department of Cellular and Molecular Medicine, University of Ottawa, Ontario, Canada. mgriffith@ogh.on.ca

Teratology
|April 1, 1997
PubMed
Summary
This summary is machine-generated.

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Midkine (MK) is crucial for the development of the secondary neural tube. Inhibiting MK expression in chick embryos led to increased neural tube defects, suggesting MK

Area of Science:

  • Developmental biology
  • Neuroscience
  • Cell biology

Background:

  • Midkine (MK) is a growth factor with known neurotrophic properties.
  • MK can induce neuroepithelial differentiation from pluripotent cells.
  • Secondary neural tube formation involves mesenchymal-neuroepithelial conversion in the tail bud.

Purpose of the Study:

  • To investigate the role of Midkine (MK) in the mesenchymal-neuroepithelial conversion process during secondary neural tube development.
  • To determine if MK is essential for proper tail bud differentiation.

Main Methods:

  • Chick embryos at the tail bud stage were treated with antisense oligodeoxynucleotides (ODNs) to inhibit MK mRNA expression.
  • Sub-blastodermal injection of ODNs was followed by reincubation for 24 or 48 hours.

Related Experiment Videos

  • MK mRNA levels were confirmed using in situ hybridization.

    • Main Results:

    • Antisense ODN treatment significantly increased the incidence of secondary neural tube defects compared to controls.
    • Control treatments included saline vehicle, sense ODNs, scrambled antisense ODNs, and non-sense ODNs.
    • Loss of MK mRNA was confirmed in treated embryos.

    Conclusions:

    • Midkine (MK) plays a significant role in the mesenchymal-neuroepithelial conversion of tail bud cells.
    • MK is essential for the proper formation of the secondary neural tube in chick embryos.