[Diagnostic test kit for detection of antibodies to syphilis agent]

A diagnostic enzyme immunoassay test kit is proposed for detecting anti-treponema antibodies in sera of syphilis patients, usable in two variants: on prefabricated polystyrene plates or by immunofiltration using a membrane filtration enzyme immunoassay kit. A peculiar feature of the test kit is the use of enzyme immunoassay conjugate based on monoclonal antibodies to light chains of human immunoglobulins, highly purified antigen from cultural T. pallidum, two substrates for detecting enzymatic activity. The efficacy of the test was confirmed on 163 patients' and control sera and compared with that of the complement fixation test, two modifications of immunofluorescent test, and micromodification of the precipitation test. The test proved to be highly sensitive, specific, and reproducible, and is recommended as a confirmation test for the diagnosis of syphilis.