An improved modification of cycle filtration leucopheresis

We report about an own modification of repetitive cycle filtration leucopheresis (RFL). Compared to the method described by De Fliedner et al. (1974) it is simpler to perform, permits the use of commercially available filtration sets and thus reduces the costs of the procedure considerably. The mean yields of the granulocytes collected in 51 RFL from unconditioned normal donors amounted from 1.7 to 2.3 X 10(10) dependent on the number of the filtration cycles performed.