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Updated: Jul 9, 2026

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing
Published on: May 8, 2014
Character of structural disturbances caused by chloroalkylamines in the model and erythrocyte membranes
O I Lebed1, V V Zhirnov, A I Luik
1Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine, Kyiv.
Abstract:
beta, beta-Dichlorodiethylamine is proved not to induce structural disturbances in phosphatidylcholine liposomes and erythrocyte membranes which is registered by fluorescence methods. Methyl-beta, beta-dichlorodiethylamine and metaxylyl-beta, beta-dichlorodiethylamine cause the increase in microviscosity of lipid bilayer hydrophobic areas in both erythrocyte membranes and liposomes. Besides, polarity of the latter also decreases, and the metaxylyl derivative alkylates nucleophilic centers of phospholipid phosphate groups in liposomes. Erythrocyte membranes, being treated by beta, beta-dichlorodiethylamine derivatives, the increase in the membrane protein hydrophobicity is registered as well as the decrease in their immersion in the lipid bilayer.
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