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A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues
J P Dalton1, S R Day, A C Drew
1Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Royal Brisbane Hospital, Australia. daltonj.@ccmail.dcu.ie
Abstract:
A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.