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Membrane changes associated with the early stages of apoptosis in HEp-2 cells decrease susceptibility to adherence by

G Cotter1, S Verhaegen, M Clynes

  • 1Department of Biology, Maynooth College, Co. Kildare, Ireland.

Journal of Medical and Veterinary Mycology : Bi-Monthly Publication of the International Society for Human and Animal Mycology
|May 1, 1997
PubMed
Summary

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Early apoptotic HEp-2 cells show reduced adherence to Candida albicans. This decreased yeast adherence correlates with loss of membrane asymmetry during early apoptosis.

Area of Science:

  • Cell Biology
  • Microbiology
  • Pathogenesis

Background:

  • Apoptosis, or programmed cell death, is a crucial biological process.
  • Candida albicans is a pathogenic yeast that can cause opportunistic infections.
  • Understanding host-pathogen interactions during apoptosis is vital for infection control.

Purpose of the Study:

  • To investigate if apoptotic HEp-2 cells exhibit altered susceptibility to Candida albicans adherence.
  • To explore the relationship between apoptosis markers and yeast binding.

Main Methods:

  • HEp-2 cells were induced into apoptosis using chemotherapeutic agents.
  • Apoptosis was confirmed via microscopy, phosphatidylserine externalization, and DNA fragmentation.
  • Adherence assays were performed using multiple Candida albicans strains.

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Main Results:

  • Early apoptotic HEp-2 cells displayed significantly reduced adherence to Candida albicans.
  • A correlation was observed between decreased yeast adherence and loss of membrane asymmetry.
  • Phosphatidylserine externalization and DNA fragmentation were markers of early apoptosis.

Conclusions:

  • Apoptosis in HEp-2 cells alters their susceptibility to Candida albicans adherence.
  • The loss of membrane asymmetry during early apoptosis is linked to reduced yeast binding.
  • These findings contribute to understanding host-pathogen dynamics in apoptotic cells.