Related Experiment Videos
Langmuir films from antibodies based on amphiphilic polyelectrolytes
J I Babitskaya1, I A Budashov, S F Chernov
1Research Center of Molecular Diagnostics and Therapy, Moscow.
Membrane & Cell Biology
|January 1, 1997
Abstract:
A technique for forming Langmuir films from antibodies based on an amphiphilic polyelectrolyte was developed. The physicochemical and immunochemical properties of the Langmuir films obtained were studied. The interaction of HBsAg with the films was found to be described by a model with one binding site, whereas that of HBsAg with antibodies adsorbed on a polystyrene plate, by a model with a positive cooperativity. The use of the novel Langmuir films from antibodies increases the sensitivity of the immunoenzyme assay.